Is this related to why 3500 Calories result in gaining or losing a pound of fat because of fat that is chemically bound to water making Calories lower than what you would get at 1 pound divided by 9 Calories per gram if you convert weight to mass and vice versa
I am wondering if maybe plants like humans also bind fat to water and if this results in fat expanding instead of shrinking when frozen
Pure fat contains approximately 9 calories per gram, which translates to roughly 4,100 calories per pound. However, body fat tissue is significantly different from pure fat. Body fat consists of adipocytes (fat cells)...
Research indicates that body fat tissue is approximately 87% fat, with the remaining 13% composed of water, protein, and other substances. This composition means the actual caloric content of body fat is considerably less than pure fat.
https://foodten.com/nutrition/how-many-calories-are-in-a-pound-of-body-fat-the-science-explained/